Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Jiang, Y.; Yu, H.; Li, F.; Cheng, L.; Zhu, L.; Shi, Y.; Gong, Q.
    Unveiling the structural features that determine the dual methyltransferase activities of Streptococcus pneumoniae RlmCD (2018), PLoS Pathog., 14, e1007379 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.189 structure of RlmCD in complex with its cofactor and the RNA substrate containing U747 at 2.00 A or U1939 at 3.10 A. The side-chain rearrangement of F145 displays an unusual mechanism through which RlmCD can discriminate between U747- and U1939-containing RNA substrate by switching the intermolecular aromatic stacking between protein and RNA on/off Streptococcus pneumoniae
2.1.1.190 structure of RlmCD in complex with its cofactor and the RNA substrate containing U747 at 2.00 A or U1939 at 3.10 A. The side-chain rearrangement of F145 displays an unusual mechanism through which RlmCD can discriminate between U747- and U1939-containing RNA substrate by switching the intermolecular aromatic stacking between protein and RNA on/off Streptococcus pneumoniae

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.189 D381A 2% residual activity towards U747 RNA substrate, 40% residual activity towards U1939 RNA substrate, reaction of EC 2.11.190 Streptococcus pneumoniae
2.1.1.189 F145A 5% residual activity towards U747 RNA substrate, 120% activity towards U1939 RNA substrate, reaction of EC 2.11.190 Streptococcus pneumoniae
2.1.1.189 H151A 5% residual activity towards U747 RNA substrate, 55% activity towards U1939 RNA substrate, reaction of EC 2.11.190 Streptococcus pneumoniae
2.1.1.189 N249A 35% residual activity towards U747 RNA substrate, 55% residual activity towards U1939 RNA substrate, reaction of EC 2.11.190 Streptococcus pneumoniae
2.1.1.189 Q162A 18% residual activity towards U747 RNA substrate, 160% activity towards U1939 RNA substrate, reaction of EC 2.11.190 Streptococcus pneumoniae
2.1.1.189 Q283A 8% residual activity towards U747 RNA substrate, 50% residual activity towards U1939 RNA substrate, reaction of EC 2.11.190 Streptococcus pneumoniae
2.1.1.189 R127A 8% residual activity towards U747 RNA substrate, 2% residual activity towards U1939 RNA substrate, reaction of EC 2.11.190 Streptococcus pneumoniae
2.1.1.190 D381A 2% residual activity towards U747 RNA substrate, reaction of EC 2.11.189, 40% residual activity towards U1939 RNA substrate Streptococcus pneumoniae
2.1.1.190 F145A 5% residual activity towards U747 RNA substrate, reaction of EC 2.11.189, 120% activity towards U1939 RNA substrate Streptococcus pneumoniae
2.1.1.190 H151A 5% residual activity towards U747 RNA substrate,reaction of EC 2.11.189, 55% activity towards U1939 RNA substrate Streptococcus pneumoniae
2.1.1.190 N249A 35% residual activity towards U747 RNA substrate, reaction of EC 2.11.189, 55% residual activity towards U1939 RNA substrate Streptococcus pneumoniae
2.1.1.190 Q162A 18% residual activity towards U747 RNA substrate, reaction of EC 2.11.189, 160% activity towards U1939 RNA substrate Streptococcus pneumoniae
2.1.1.190 Q283A 8% residual activity towards U747 RNA substrate, reaction of EC 2.11.189, 50% residual activity towards U1939 RNA substrate Streptococcus pneumoniae
2.1.1.190 R127A 8% residual activity towards U747 RNA substrate, reaction of EC 2.11.189, 2% residual activity towards U1939 RNA substrate Streptococcus pneumoniae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.189 additional information Streptococcus pneumoniae enzyme introduces C5 methylation at both U747 and U1939 of the 23S ribosomal RNA, i. e. catalyzes both the reactions of EC 2.18189 and 2.11.190 ?
-
-
2.1.1.189 additional information Streptococcus pneumoniae ATCC BAA-334 enzyme introduces C5 methylation at both U747 and U1939 of the 23S ribosomal RNA, i. e. catalyzes both the reactions of EC 2.18189 and 2.11.190 ?
-
-

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.189 Streptococcus pneumoniae Q97R12 introduces C5 methylation at both U747 and U1939 of the 23S ribosomal RNA, i. e. catalyzes both the reactions of EC 2.1.1.189 and EC 2.1.1.190; serotype 4
-
2.1.1.189 Streptococcus pneumoniae ATCC BAA-334 Q97R12 introduces C5 methylation at both U747 and U1939 of the 23S ribosomal RNA, i. e. catalyzes both the reactions of EC 2.1.1.189 and EC 2.1.1.190; serotype 4
-
2.1.1.190 Streptococcus pneumoniae Q97R12 introduces C5 methylation at both U747 and U1939 of the 23S ribosomal RNA, i. e. catalyzes both the reactions of EC 2.1.1.189 and EC 2.1.1.190
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.189 additional information enzyme introduces C5 methylation at both U747 and U1939 of the 23S ribosomal RNA, i. e. catalyzes both the reactions of EC 2.18189 and 2.11.190 Streptococcus pneumoniae ?
-
-
2.1.1.189 additional information enzyme introduces C5 methylation at both U747 and U1939 of the 23S ribosomal RNA, i. e. catalyzes both the reactions of EC 2.1.1.189 and 2.1.1.190 Streptococcus pneumoniae ?
-
-
2.1.1.189 additional information enzyme introduces C5 methylation at both U747 and U1939 of the 23S ribosomal RNA, i. e. catalyzes both the reactions of EC 2.18189 and 2.11.190 Streptococcus pneumoniae ATCC BAA-334 ?
-
-
2.1.1.189 additional information enzyme introduces C5 methylation at both U747 and U1939 of the 23S ribosomal RNA, i. e. catalyzes both the reactions of EC 2.1.1.189 and 2.1.1.190 Streptococcus pneumoniae ATCC BAA-334 ?
-
-
2.1.1.190 additional information enzyme introduces C5 methylation at both U747 and U1939 of the 23S ribosomal RNA, i. e. catalyzes both the reactions of EC 2.1.1.189 and 2.1.1.190 Streptococcus pneumoniae ?
-
-
2.1.1.190 additional information enzyme introduces C5 methylation at both U747 and U1939 of the 23S ribosomal RNA, i. e. catalyzes both the reactions of EC 2.1.1.189 and 2.1.1.190 Streptococcus pneumoniae ATCC BAA-334 ?
-
-

Synonyms

EC Number Synonyms Comment Organism
2.1.1.189 RlmCD
-
 Streptococcus pneumoniae
2.1.1.189 SP_1029
-
 Streptococcus pneumoniae
2.1.1.190 RlmCD
-
 Streptococcus pneumoniae
2.1.1.190 SP_1029
-
 Streptococcus pneumoniae